Double antibody separation has been commonly used since the earliest days of radioimmunoassay (RIA)1,2 and is very reliable. Less demanding procedures have been sought, in particular solid phase antibody techniques3,4.
Sac-Cel®, which is antibody covalently coupled to microfine cellulose particles, successfully combines the specificity of liquid antibody with the speed, simplicity and precision of solid phase separation, and has been adopted by a number of kit manufacturers, including IDS. Sac-Cel® has also been used in affinity purification5. Two different species types are currently available: anti-rabbit and anti-sheep/goat.
1. Hales, C.N. and Randle, P.J. (1963). Biochem. J., 88, 137.
2. Koninckx, Ph., Bouillon, R. and De Moor, P. (1976). Acta Endocr. (Kbh)., 81, 45-53.
3. Morgan, C.R. and Lazarow, A. (1963). Diabetes, 12, 115.
4. Sluiter, W.J. et al. (1972). Clin. Chim. Acta., 42, 255.
5. Gibson, J.M. Aplin, J.D. White A. and Westwood M. (2001). Mol. Hum. Reprod., 7: 79-87.