- Assessment of Bone Resorption in Rodents 1, 2, 3
- Ovariectomised (OVX) rats and mice
- Thyroidparathyroidectomised (TPTX) rats
- Rat and mouse models of bone metastases
- Knock-out and transgenic mice with effect in bone resorption
- Assessment of Bone Resorption in Tissue Culture
- Calvariae, tibiae, or metatarsals from rats and mice
- Multi-disciplinary use -measuring urine and serum samples
- All reagents ready for use
- A complete assay panel supporting bone research
RatLaps™ (CTX-I) EIA is an enzyme-linked immunosorbent assay for the quantitative determination of bone-related degradation products from C-terminal telopeptides of type I collagen in rat/mouse serum or urine, and from rat/mouse bone released into cell culture supernatants by osteoclasts. The assay is for research use only.
Type I collagen accounts for more than 90% of the organic matrix of bone and is synthesised primary in bone.
During renewal of the skeleton bone matrix is degraded and consequently fragments of type I collagen are released into circulation. The RatLaps™ (CTX-I) EIA is based on the observation that certain C-telopeptide degradation products from type I collagen released during osteoclastic bone resorption.
The RatLaps™ (CTX-I) EIA is based upon the competitive binding of a polyclonal antibody to soluble RatLaps antigens EKSQDGGR or to immobilised RatLaps antigens. Briefly, the polyclonal antibody is raised against a synthetic peptide having a sequence (EKSQDGGR) specific for a part of the C-terminal telopeptide α1 chain of rat type I collagen.
For standardisation of the RatLaps™ (CTX-I) EIA a synthetic peptide (EKSQDGGR) that is specific for the C-terminal telopeptide α1 chain of type I collagen in rats has been used.
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