• Fully automated, spectrophotometric Angiotensin Converting Enzyme assay
  • Less than 15 minutes to a direct and quantitative first result
  • Accurate results for ACE concentrations in serum samples
  • Complete reagent package including calibrator and controls
  • Laboratories can provide clinicians with the tools to assist in the monitoring of diagnosed sarcoidosis

The IDS ACE is an in vitro diagnostic enzymatic assay device intended for the quantitative determination of ACE (Angiotensin Converting Enzyme) in human serum on the IDS System.

Angiotensin Converting Enzyme catalyses the conversion of the inactive decapeptide Angiotensin I to Angiotensin II. ACE is a membrane-bound enzyme found on various cell types, including vascular endothelial cells, renal proximal tubule cells, and neuroepithelial cells1.

ACE is also known as kininase II and metabolizes a number of other peptides, including the vasodilator peptides bradykinin and kallidin2. Functionally, the actions of ACE potentially result in increased vasoconstriction and decreased vasodilation.

The kinetics of such a reaction can be measured by recording the decrease in absorbance at 340 nm3,4, a method which led to the possibility of a direct spectrophotometric assay for serum ACE levels. The fully automated IDS ACE kinetic method is standardized with a colorimetric kit according to the described reference method5,6.

Interest in serum ACE levels began with the observation that levels are increased in approximately 60% of patients with sarcoidosis, an inflammatory disease of unknown origin characterized by the formation of granuloma7,8. Many other conditions have also been shown to be associated with altered ACE levels, however monitoring of response to treatment in sarcoidosis seems to be the most useful role of the assay9. Some reports exist describing differences in ACE levels according to age and gender, with children between 4-18 years having higher ACE concentrations than adults, however most authors have not noted any differences between males and females. ACE values can vary widely between individuals, mainly due to genetic deletion-/insertion-polymorphisms of the ACE gene.

Chronic forms of Sarcoidosis can last several years and therefore appropriate diagnostic tools are essential to closely monitor disease activity and to predict disease progression.

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  3. Ronca-Testoni S.: Direct spectrophotometric assay for angiotensin-converting enzyme in serum. Clin Chem 29, 1093-1096 (1983).
  4. Bénéteau B. and Baudin B. et al.: Automated kinetic assay of angiotensin-converting enzyme in serum. Clin Chem 32, 884-886 (1986).
  5. Lieberman J.: Elevation of serum angiotensin converting enzyme (ACE) level in Sarcoidosis. Am J Med 59, 36-72 (1975).
  6. Hurst PL. and Lovell-Smith CJ.: Optimized assay for serum angiotensin converting enzyme activity. Clin Chem 27, 2048-2052 (1981).
  7. Studdy PR. and Bird R. Serum angiotensin-converting enzyme in Sarcoidosis – its value in present clinical practice. Ann Clin Biochem 26, 13-18 (1990).
  8. Iannuzzi MC. Et al.: Sarcoidosis. N Engl J Med 357, 2153-65 (2007).
  9. Muller BR. Analysis of serum angiotensin-converting enzyme. Ann Clin Biochem 39, 436-443 (2002).