Unique Features

  • Unique and proprietary immunocapsules – simplifies the sample purification procedure and eliminates the need for organic solvents
  • Enzymatic immunoassay – eliminates radioactive waste
  • Cost-effective, eliminates the need for organic solvents
  • Unique and proprietary immunocapsules – simplifies the sample purification procedure

The 1,25-Dihydroxy Vitamin D EIA kit is a complete assay system intended for the purification of 1,25-dihydroxyvitamin D [1,25D] in human serum or plasma by immunopurification followed by quantification by enzymatic immunoassay to assess 1,25-dihydroxyvitamin D deficiency associated with renal disease.

1,25-dihydroxyvitamin D (1,25(OH)2D) is the biologically active metabolite of vitamin D that plays a key role in the regulation of calcium and phosphate homeostasis.

Measurement of 1,25(OH)2D is important in managing patients with renal failure, hyperphosphataemia, hypomagnesaemia, hypoparathyroidism, pseudohyperparathyroidism, vitamin D-dependent rickets, granulomatous disease, lymphoma, primary hyperparathyroidism, and hypercalcaemia.

Endres DB, Rude RK. Mineral and bone metabolism. In: Burtis CA, Ashwood ER, Bruns DE, editors. Tietz textbook of clinical chemistry and molecular diagnostics. St. Louis: Elsevier Saunders; 2006. p. 1891–965.

Holick, MF., et al. “Evaluation, treatment, and prevention of vitamin D deficiency: an Endocrine Society clinical practice guideline.” The Journal of Clinical Endocrinology & Metabolism 96.7 (2011): 1911-1930.

Seiden-Long, Isolde, and Reinhold Vieth. “Evaluation of a 1, 25-dihydroxyvitamin D enzyme immunoassay.” Clinical chemistry 53.6 (2007): 1104-1108.