- Direct urine measurement, no extraction procedure required, for streamlined workflow
- No risk of biotin interference by employing direct antibody-solid phase coating principle
- Excellent functional sensitivity and precision over the clinically relevant range
- Validated reference intervals in 24-hour urine collections from healthy subjects
- Demonstrated clinical concordance in routine clinical samples
The IDS Urinary Cortisol assay is a fully automated CLIA assay for the quantitative determination of cortisol in human urine samples. Results are to be used in conjunction with other clinical and laboratory data to assist clinicians in the diagnosis and monitoring of disorders of the adrenal gland.
Cortisol is a steroid hormone synthesized in the adrenal gland regulating a variety of physiological processes 1,2,3. The IDS Urinary Cortisol results are to be used in conjunction with other clinical and laboratory data to assist clinicians in the diagnosis and monitoring of hypercortisolism, commonly referred to as Cushing’s syndrome4.
Urine free cortisol (UFC) represents free serum cortisol levels which are not bound to the cortisol-binding-globulin (CBG) and are filtered into urine. Approximately 1% of unbound cortisol is excreted in the urine5. UFC has the advantage of not being affected by conditions that alter CBG levels such as oral contraceptives.
The Endocrine Society recommends the measurement of 24hr urine free cortisol as one of the screening tests for diagnosing Cushing’s syndrome6, with a cut-off point for a normal response considered the upper limit of normal (ULN) range as established for each respective assay.
- Ramamoorthy, S. & Cidlowski, J. A. Corticosteroids: Mechanisms of Action in Health and Disease. Rheumatic diseases clinics of North America 42, 15-31, (2016).
- Wild, D. The immunoassay handbook. (Elsevier, 2005).
- Cain, D. W. & Cidlowski, J. A. Specificity and sensitivity of glucocorticoid signalling in health and disease. Best practice & research. Clinical endocrinology & metabolism 29, 545-556, (2015).
- Findling, J. W. & Raff, H. Cushing’s Syndrome: important issues in diagnosis and management. The Journal of clinical endocrinology and metabolism 91, 3746-3753, (2006).
- Raff, H. et al. Urine free cortisol in the diagnosis of Cushing’s syndrome: Is it worth doing and, if so, how? The Journal of clinical endocrinology and metabolism 100(2), 395-397, (2015).
- Nieman, L. K. et al. The diagnosis of Cushing’s syndrome: an Endocrine Society Clinical Practice Guideline. The Journal of clinical endocrinology and metabolism 93, 1526-1540, (2008).
IDS Salivary Cortisol
IDS Direct Renin