Unique Features

  • Unique – the first commercially available EIA  to quantify type I collagen formation in rat or mouse specimens:
  • Ideal for rodents – only 5μL per well
  • Flexible – serum or plasma samples can be used
  • Fast turnaround time – results in 3 hours
  • Complementary – use in conjunction with IDS RatTRAPTM
  • MouseTRAPTM or RatLapsTM to evaluate both components of bone remodelling
  • A complete assay panel supporting bone research
  • Low sample volume – only 5μL per well
  • Multi-disciplinary use – measuring plasma and serum samples

The Immunodiagnostic Systems Limited Rat/Mouse PINP EIA kit is a competitive enzyme immunoassay (EIA) for the quantitative determination of N-terminal propeptide of type I procollagen (PINP) in rat or mouse serum or plasma samples.

PINP is a particularly useful marker for monitoring the efficacy of osteoporosis therapy with anabolic agents, but it is also one of the best bone turnover markers for monitoring the efficacy of antiresorptive therapy.

The Rat/Mouse PINP EIA assay is a specific method to determine PINP released during rat and mouse bone collagen synthesis, and it has no cross-reactivity for human PINP.

The Rat/Mouse PINP EIA assay can be used for determining the bone formation rate from rat and mouse serum samples1, 2, 3, and from the culture medium in rat and mouse osteoblast cultures.

Previous studies have demonstrated that PINP secretion is increased after stimulation of mouse osteoblasts by BMP-4 and oestrogen in vitro. PINP values are also elevated after treatment with PTH in both young ovariectomised rats and old intact rats.

Rissanen JP et al., Short-term changes in serum PINP predict long-term changes in trabecular bone in the rat ovariectomy model. Calcif Tissue Int. 2008 Feb;82(2):155-61.

Hale LV et al., PINP: a serum biomarker of bone formation in the rat. Bone. 2007 Apr;40(4):1103-9.

Jia T et al., A novel model of adenine-induced tubulointerstitial nephropathy in mice. BMC Nephrol. 2013 May 30;14:116.