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BoneTRAP® (TRAcP 5b) ELISA

Disease Areas: Bone metabolism

Format: ELISA

Description

High amount of tartrate-resistant acid phosphatase (TRACP) is expressed by bone resorbing osteoclasts and activated macrophages1. Two forms of TRACP circulate in human blood, known as TRACP 5a and TRACP 5b2. TRACP 5b is derived from osteoclasts and TRACP 5a from macrophages3.

Features

  • Measures TRACP 5b activity that is released specifi cally from osteoclasts.
  • No interference with TRACP 5a or other phosphatases.
  • Hemolysis has no effect on results.
  • No diurnal variation.
  • Not affected by functional disorders of kidney and liver.
  • No dietary influences.

Assay Details

Format96 Wells
Code
SB-TR201A
MethodologyELISA
Calibrators
6
Controls2
Assay Range0.5 to 10 U/L
Specimen TypeSerum, EDTA plasma
Sensitivity0.5 U/L
Specimen Volume100 µL
Incubation time60 min. at Room Temp.

References

  1. Yaziji H, Janckila AJ, Lear SC, Martin AW, Yam LT 1995 Immunohistochemical detection of tartrate-resistant acid phosphatase in non-hematopoietic human tissues. Am J Clin Pathol 104:397-402.
  2. Lam KW, Li CY, Yam LT, Desnick RJ 1981 Comparison of the tartrate-resistant acid phosphatase in Gaucher’s disease and leukemic reticuloendotheliosis. Clin Biochem 14:177-181.
  3. Janckila AJ, Neustadt DH, Nakasato YR, Halleen JM, Hentunen T, Yam LT 2002 Serum tartrate-resistant acid phosphatase isoforms in rheumatoid arthritis. Clin Chim Acta. 320:49-58.

Product information correct as of 2nd August 2012