1,25-Dihydroxy Vitamin D RIA
Disease Areas: Bone metabolism Vascular calcification Cartilage metabolism
Format: RIA
Description
The IDS 1,25-Dihydroxy Vitamin D RIA kit is a complete assay system intended for the purification of 1,25-dihydroxyvitamin D (1,25D) in human serum or plasma by immunoextraction followed by quantitation by 125I radioimmunoassay. Results are to be used in conjunction with other clinical and laboratory data to assist the clinician in the assessment of 1,25D deficiency associated with renal disease in adult populations.
Features
- Unique Immunocapsule - replaces tedious, multi-solvent C18 extraction procedures.
- Simplidied procedure - saves technician time and eliminates need for extraction instrumentation.
- Small sample volume - ideal for Paediatrics.
- Quantitative recocery of 1,25-Dihydroxy Vitamin D - No need for individual sample recovery determination and correction.
- Co-Specific - for 1,25-(OH)2 D2 and 1,25-(OH)2 D3 with <0.01% cross-reactivity with 25-(OH) D.
Assay Details
| Code | AA-54F1/AA-54F2 |
| Methodology | RIA |
| Format | 40 Columns/56 Columns |
| Number of Tests per Kit | 18/26 determinations in duplicate |
| Calibrators | 6 (not fixed values) |
| Controls | 2 |
| Specimen Type | Serum or plasma |
| Specimen Volume | <500µL (alternative protocol exists) |
| Sample Extraction | Yes |
| Sensitivity | 5 pmol/L (2.1 pg/mL) |
| Assay Range | Approx. 8 - 500 pmol/L |
| Samples Preperation | Immunocapsules (reagents included) |
| Incubation Time | Overnight. at 2-8°C |
Product information correct as of 2nd September 2011